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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
16/03/2017 |
Data da última atualização: |
03/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZILAGYI -ZECCHIN, V. J. S.; ADAMOSKI, D.; GOMES, R. R.; HUNGRIA, M.; IKEDA, A. C.; KAVA -CORDEIRO, V.; GLIENKE, C.; GALLI -TERASAWA, L. V. |
Afiliação: |
VIVIAN J. S ZILAGYI-ZECCHIN, UFPR; DOUGLAS ADAMOSKI, UFPR; RENATA RODRIGUES GOMES, UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO; ANGELA C. IKEDA, UFPR; VANESSA KAVA -CORDEIRO, UFPR; CHIRLEI GLIENKE, UFPR; LYGIA V. GALLI-TERASAWA, UFPR. |
Título: |
Composition of endophytic fungal community associated with leaves of maize cultivated in south brazilian field. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Acta Microbiologica et Immunologica Hungarica, v. 63, n. 4, p. 449-466, 2016. |
ISSN: |
1588-2640 |
DOI: |
10.1556/030.63.2016.020 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola , and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe /Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages. |
Palavras-Chave: |
Microbiota. |
Thesagro: |
Fungo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01934naa a2200253 a 4500 001 2067198 005 2017-11-03 008 2016 bl uuuu u00u1 u #d 022 $a1588-2640 024 7 $a10.1556/030.63.2016.020$2DOI 100 1 $aZILAGYI -ZECCHIN, V. J. S. 245 $aComposition of endophytic fungal community associated with leaves of maize cultivated in south brazilian field.$h[electronic resource] 260 $c2016 520 $aThe objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola , and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe /Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages. 650 $aFungo 653 $aMicrobiota 700 1 $aADAMOSKI, D. 700 1 $aGOMES, R. R. 700 1 $aHUNGRIA, M. 700 1 $aIKEDA, A. C. 700 1 $aKAVA -CORDEIRO, V. 700 1 $aGLIENKE, C. 700 1 $aGALLI -TERASAWA, L. V. 773 $tActa Microbiologica et Immunologica Hungarica$gv. 63, n. 4, p. 449-466, 2016.
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Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Alimentos e Territórios. |
Data corrente: |
17/11/2023 |
Data da última atualização: |
17/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
PINHEIRO, J. C. A.; SOUZA, C. E. S. DE; RIBEIRO, D. A.; SILVA, A. DE A.; SILVA, V. B. DA; SANTOS, A. T. L. DOS; FONSECA, V. J. A.; MACÊDO, D. G. DE; CRUZ, R. P. DA; BEZERRA, J. W. A.; MACHADO, A. J. T.; FREITAS, T. S. DE; BRITO, E. S. de; RIBEIRO, P. R. V.; COSTA, J. G. M. DA; COUTINHO, H. D. M.; KOWALSKA, G.; ROWINSKI, R.; KOWALSKI, R.; BRAGA, M. F. B. M. |
Afiliação: |
JACQUELINE COSMO ANDRADE-PINHEIRO, Regional University of Cariri; CELESTINA ELBA SOBRAL DE SOUZA, Regional University of Cariri; DAIANY ALVES RIBEIRO, Regional University of Cariri; ANDRESSA DE ALENCAR SILVA, Regional University of Cariri; VIVIANE BEZERRA DA SILVA, Federal University of Pernambuco; ANTONIA THASSYA LUCAS DOS SANTOS, Regional University of Cariri; VICTOR JUNO ALENCAR FONSECA, Regional University of Cariri; DELMACIA GONÇALVES DE MACÊDO, Regional University of Cariri; RAFAEL PEREIRA DA CRUZ, Regional University of Cariri; JOSÉ WEVERTON ALMEIDA-BEZERRA, Regional University of Cariri; ANTONIO JÚDSON TARGINO MACHADO, Regional University of Cariri; THIAGO SAMPAIO DE FREITAS, Regional University of Cariri; EDY SOUSA DE BRITO, CNAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; JOSÉ GALBERTO MARTINS DA COSTA, Regional University of Cariri; HENRIQUE DOUGLAS MELO COUTINHO, Regional University of Cariri; GRAZYNA KOWALSKA, University of Life Sciences in Lublin; RAFAL ROWINSKI, University of Life Sciences in Lublin; RADOSLAW KOWALSKI, University of Life Sciences in Lublin; MARIA FLAVIANA BEZERRA MORAIS-BRAGA, Regional University of Cariri. |
Título: |
LC-MS analysis and antifungal activity of Turnera subulata Sm. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Plants, v. 12, n. 415, 2023. |
Páginas: |
12 p. |
DOI: |
10.3390/plants12020415 |
Idioma: |
Inglês |
Conteúdo: |
Fungi of the Candida genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as Turnera subulata become attractive to research. The present work aimed to analyze the ethanol extract of Turnera subulata leaves against standard strains of Candida albicans, Candida krusei and Candida tropicalis using broth microdilution techniques. The identification of the compounds in T. subulata leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of C. albicans and C. tropicalis. It is possible that the T. subulata extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors. |
Palavras-Chave: |
Antifungal; Antifúngico; Extract; Extrair; Levedura; Virulência. |
Thesaurus NAL: |
Antifungal agents; Extracts; Virulence; Yeasts. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158504/1/LC-MS-Analysis.pdf
|
Marc: |
LEADER 02755naa a2200493 a 4500 001 2158504 005 2023-11-17 008 2023 bl uuuu u00u1 u #d 024 7 $a10.3390/plants12020415$2DOI 100 1 $aPINHEIRO, J. C. A. 245 $aLC-MS analysis and antifungal activity of Turnera subulata Sm.$h[electronic resource] 260 $c2023 300 $a12 p. 520 $aFungi of the Candida genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as Turnera subulata become attractive to research. The present work aimed to analyze the ethanol extract of Turnera subulata leaves against standard strains of Candida albicans, Candida krusei and Candida tropicalis using broth microdilution techniques. The identification of the compounds in T. subulata leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of C. albicans and C. tropicalis. It is possible that the T. subulata extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors. 650 $aAntifungal agents 650 $aExtracts 650 $aVirulence 650 $aYeasts 653 $aAntifungal 653 $aAntifúngico 653 $aExtract 653 $aExtrair 653 $aLevedura 653 $aVirulência 700 1 $aSOUZA, C. E. S. DE 700 1 $aRIBEIRO, D. A. 700 1 $aSILVA, A. DE A. 700 1 $aSILVA, V. B. DA 700 1 $aSANTOS, A. T. L. DOS 700 1 $aFONSECA, V. J. A. 700 1 $aMACÊDO, D. G. DE 700 1 $aCRUZ, R. P. DA 700 1 $aBEZERRA, J. W. A. 700 1 $aMACHADO, A. J. T. 700 1 $aFREITAS, T. S. DE 700 1 $aBRITO, E. S. de 700 1 $aRIBEIRO, P. R. V. 700 1 $aCOSTA, J. G. M. DA 700 1 $aCOUTINHO, H. D. M. 700 1 $aKOWALSKA, G. 700 1 $aROWINSKI, R. 700 1 $aKOWALSKI, R. 700 1 $aBRAGA, M. F. B. M. 773 $tPlants$gv. 12, n. 415, 2023.
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